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  Indian J Med Microbiol
 

Figure 1: Primary culture of sheep and rat keratinocytes (a) Diagram showing follicle compartments dissected for explant and culture (epithelial tissue, yellow; dermal tissue, red); (b) Intact DP/GE explant immunostained for PCNA to label proliferating cells.Expression (black) is restricted to fragments of GE tissue surrounding the junction between the DP (upper) and DS (lower); (c) Photomontage of primary cells growing from an ovine DP/GE explant (phase contrast view).The original explant is in the center of the image.Two cell types can be seen growing out from it— polygonal keratinocytes clustered on the left side of the colony and scattered, stellate dermal cells concentrated toward the upper right.Note that this colony has proportionally more dermal cells than is typical; (d) K14 immunostaining of an ovine vibrissa DP/GE colony. K14 expression is seen as green, nuclei are counterstained with DAPI (blue); (e) Higher power view of a second ovine vibrissa DP/GE colony immunostained for K14.Labeled keratinocytes can be seen on the right of the image, with scattered, unlabelled dermal cells on the left; (f) DIC image of the field of view shown in (e); (g, h) Two primary cultures of cells grown from rat DP/GE explants, immunostained for K14 expression (green), nuclei counterstained with DAPI (blue).K14-positive cells are less frequent and more variable in morphology compared with ovine cultures; (i, j) Two primary cultures of cells grown from rat DS/ORS explants, immunostained for K14 expression (green), nuclei counterstained with DAPI (blue). K14-positive cells are similar in abundance and morphology to ovine cultures.In all images, scale bars = 100 μm

Figure 1: Primary culture of sheep and rat keratinocytes (a) Diagram showing follicle compartments dissected for explant and culture (epithelial tissue, yellow; dermal tissue, red); (b) Intact DP/GE explant immunostained for PCNA to label proliferating cells.Expression (black) is restricted to fragments of GE tissue surrounding the junction between the DP (upper) and DS (lower); (c) Photomontage of primary cells growing from an ovine DP/GE explant (phase contrast view).The original explant is in the center of the image.Two cell types can be seen growing out from it— polygonal keratinocytes clustered on the left side of the colony and scattered, stellate dermal cells concentrated toward the upper right.Note that this colony has  proportionally more dermal cells than is typical; (d) K14 immunostaining of an ovine vibrissa DP/GE colony. K14 expression is seen as green, nuclei are counterstained with DAPI (blue); (e) Higher power view of a second ovine vibrissa DP/GE colony immunostained for K14.Labeled keratinocytes can be seen on the right of the image, with scattered, unlabelled dermal cells on the left; (f) DIC image of the field of view shown in (e); (g, h) Two primary cultures of cells grown from rat DP/GE explants, immunostained for K14 expression (green), nuclei counterstained with DAPI (blue).K14-positive cells are less frequent and more variable in morphology compared with ovine cultures; (i, j) Two primary cultures of cells grown from rat DS/ORS explants, immunostained for K14 expression (green), nuclei counterstained with DAPI (blue). K14-positive cells are similar in abundance and morphology to ovine cultures.In all images, scale bars = 100 μm